https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/873 Sun, 12 Jul 2026 22:35:07 GMT 2026-07-12T22:35:07Z https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/895 Establishment of Anticancer Potentiality of New Conjugated Fatty Acid(S) Extracted from Tapra Fish (Opisthopterus tardoore) Oil: A Mechanistic Study. Dutta, Ananya It has been demonstrated that Linoelaidic acid, an isomer of omega-6 fatty acid, can prevent cancer both in vivo and in vitro by decreasing cell viability, increasing oxidative stress, causing DNA fragmentation, and inducing cell cycle arrest. Linoelaidic acid is obtained from the oil of Tapra fish (Opisthopterus tardoore). Breast cancer, the most common disease among women worldwide, is a major public health concern that impacts individuals and communities everywhere, but particularly in low- and middle-income countries. Even though there are efficient therapies and early detection techniques, there are still issues with disparities in access to care, different survival rates, and the disease's many risk factors. In order to lessen the burden of the disease and enhance results, societal efforts concentrate on early identification, prompt diagnosis, thorough management, and lowering risk factors through lifestyle modifications. There are many type of medication and chemotherapeutic treatments available in market for cancer treatment but these treatments are very expensive, time consuming, less effective and not free from the side effects and also not an ultimate treatment of cancer. Towards alternative treatment strategies, this Ph.D. work was aimed to establish an innovative approaches to manage the cancer by supplementation of Linoelaidic acid in both in vivo and in vitro which might be shown increment of apoptosis rate in cancer by covering the basic physiological process for the management of cancer by noting the upregulation of p53 on cell proliferation inhibition and DNA fragmentation. To achieve the ultimate aim and objectives of the thesis, at first the Linoelaidic acid was identified after isolation and characterization of Tapra fish (Opisthopterus tardoore) oil and also check out the purification of Linoelaidic acid by using HPLC. For checking the edibility of this fish oil physicochemical properties was checked. Second experiment was designed to check the anticancer potentiality of Linoelaidic acid by checking cell viability, cell cytotoxicity, intracellular redox balance, and checked different gene expression of both ER positive(MCF-7) & ER negative (MDA-MB-231) human breast cancer cell line after treatment with linoelaidic acid in different doses (2µM/ml, 5µM/ml, 10µM/ml) and also checked the effect of Doxorubicin (5µM/ml) on MCF-7 and MDA-MB-231 human breast cancer cell line. Third experiment was designed to check if there are any adverse effect of Linoelaidic acid on Peripheral Blood Lymphocyte (PBLs) which is known as a normal blood cell. This experiment demonstrated that both Linoelaidic acid and Doxorubicin was applied on Peripheral Blood Lymphocyte (PBLs) as per same doses applied on MCF-7 and MDA-MB-231 human breast cancer cell line by checking cell viability, cell cytotoxicity, intracellular redox balance measurement and responsible gene marker of apoptosis was checked. In that case the Doxorubicin shows adverse effect on Peripheral Blood Lymphocyte (PBLs) but there is no hazardous effect find out on PBLs after supplementation with Linoelaidic acid. In fourth experiment of this work explained about the mechanism of apoptosis induces by Linoelaidic acid on both MCF-7 and MDA-MB-231 human breast cancer cell line by Immunoblotting, morphological changes of these two types of breast cancer cell line and DNA fragmentation. After completion of in vitro study we applied Linoelaidic acid on in vivo (C57BL 6J) model in fifth experiment. Mice were separated in 4 groups, and injected with MDA-MB-231 cancer cell at 5×106 cell/100g body weight. After 15 days treatment group I, II and III were treated with Linoelaidic acid at selected dose up to 56 days. After sacrifice tumor size was measured, single cell prepared from tumor tissue and morphological changes was checked by using DAPI staining, AO/Et-Br dual staining, and DNA fragmentation by TUNEL assay. The overall findings and outcomes from all the experiment, it has been proved that Linoelaidic acid mitigates the cell viability in both in vivo and in vitro group without hampering the normal cell. Linoelaidic acid helps to increase the mitochondrial dysfunction for increase the oxidative stress to initiate apoptosis. So that it may be established that it is an innovative approach that the source of linoelaidic acid is cheap, more convenient and harmless therapy and people can easily consume this type of fatty acid though their daily diet as food component to prevent or manage breast cancer. This Tapra fish (Opisthopterus tardoore) is easily available in the southern part of West Bengal. All type of community people can easily effort this fish as their daily diet. Fri, 10 Jul 2026 00:00:00 GMT https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/895 2026-07-10T00:00:00Z https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/894 Representation of Indigeneity in the Folk Narratives of Select North-East Indian Tribes: A Study in Environmental Posthumanities. Chakravorty, Abhishek This thesis has attempted to explore how Indigeneity and Environmental Posthumanities can intersect effectively for sake of a planetary future where existence is shaped by mutual recognition of human and nonhuman agencies. The emphasis has been given on the folk narratives of the three major indigenous tribal communities from North-East India- the Lepchas, Garos and Khasis. North-East India has always been a powerful biodiversity hotspot. At the same time, it is also a socio-cultural hotspot. The indigenous tribes and their cultural representations carry effective portrayals of relationality where all members, human and nonhuman, of the ecological community coexist together benefitting each other. The folk narratives successfully counter the anthropocentric worldviews where man is at the center of the environmental structure. The emphasis is given on generating an ethical treatment toward land and water bodies that constitute indigenous tribal geological models of the Lepchas, Garos and Khasis. Animals and vegetal bodies are given agencies in the folk narratives of the three tribes. They have been portrayed as vital members of the ecological community sharing same value as human beings. The folk narratives also represent the supernatural beliefs of the three tribal communities as major environmental metaphors and tools that structure a posthuman turn. The folk narratives of the Lepchas, Garos and Khasis carry the essence of their indigenous identities which effectively parallel with the ideologies of Environmental Posthumanities to develop a single vision of planetary coexistence and survival. Fri, 10 Jul 2026 00:00:00 GMT https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/894 2026-07-10T00:00:00Z https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/893 Anti-Obesity and Anti-Gastric Ulcer Activity of Probiotic Lactic Acid Bacterium Isolated from Traditional Fermented Foods. Das, Tridip Kumar Lactic acid bacteria (LAB) have garnered significant attention for their remarkable probiotic potential, which plays a vital role in promoting gut health and enhancing overall well-being. Beneficial microorganisms, particularly LAB, are primarily present in traditional fermented foods, which represent a rich source of diverse strains of LAB. Each strain possesses distinct functional properties that can positively influence human health. The aim of this study was to evaluate the anti-obesity and anti-gastric ulcer activity of probiotic lactic acid bacterium isolated from traditional fermented foods. Considering this, a comprehensive isolation process was conducted, resulting in the isolation of 12 distinct lactic acid bacterial colonies from a variety of fermented products, including idli batter, dosa batter, and haria (a rice-based fermented beverage). The standard plate method was employed for LAB isolation using Rogosa SL agar media (supplemented with 0.132% acetic acid). The morphological analysis confirmed that all strains were rod-shaped, Gram-positive, non-motile, and non-spore-forming. The negative test for Indole, Methyl Red, Voges-Proskauer, and citrate utilization confirmed their reliance on carbohydrate fermentation. The enzymatic analysis revealed that the isolates might exhibit significant α-amylase, glucoamylase, and protease activity. Further in vitro probiotic characterization of the isolated LAB was conducted. Based on the cumulative probiotic score, we selected the E2_MCCKT strain for further investigation. After 120 min of incubation, the E2_MCCKT strain exhibited the highest survivability at different pH levels. The survival rate of the E2_MCCKT strain was 98.4% at pH 2, 99.3% at pH 3, and 100% at pH 6.8, which was statistically nonsignificant. Similarly, the E2_MCCKT strain demonstrated significant resistance to bile salts and could endure 0.3% and 2% bile salt concentrations for 120 min. The survival rate of the strain was 98.8% in 0.3% bile and 97.69% in 2% bile salt after 120 min of incubation. Concurrently, the E2_MCCKT strain exhibited moderate autoaggregation ability (52.52%) and surface hydrophobicity (38.08%), which was higher in comparison to other isolates. The antibiotic susceptibility tests indicated that the bacterium had intermediate sensitivity to ampicillin and chloramphenicol. The bacterium was identified as Lactiplantibacillus plantarum E2_MCCKT through 16S rRNA gene sequence analysis. E2_MCCKT exhibited non-haemolytic activity, making it a safe candidate for probiotic applications. Subsequently, E2_MCCKT was tested on obese mice to demonstrate its potential anti-obesity effects. The initial body weight for all male albino mice was 15.39±0.19g, and they were acclimated for 10 days in standard conditions (32±2°C and 50% humidity) with a 12-hour light/dark cycle. The mice were provided with regular food and free access to water. Mice were randomly selected and divided into three groups (n=5) based on the food and treatment. The first group, known as the normal diet (ND), was fed a meal consisting of foods with a ratio of carbohydrate, protein, and fat (64.2:22.3:13.5). The second group was the high-fat diet (HFD), which received lab-made food with a ratio of carbohydrates, protein, and fat (38.9:22.2:38.9). The high-fat treatment group (HFT) was the final group; it received a highfat diet for 8 weeks and probiotic Lp. plantarum E2_MCCKT (109 CFU/ml) for the final 4 weeks. The HFT group received treatment for the probiotic bacteria four weeks following the onset of obesity. The final average body weight and BMI were found to be high in HFD mice after the eightweek investigation period. Furthermore, the HFD group had the greatest levels of total serum cholesterol, triglycerides, LDL, and VLDL when compared to the other groups. In contrast, the Lp. plantarum E2_MCCKT treatment was able to reduce these parameters drastically, and the results were comparable to those of the ND group. The HFD group had higher SGOT and SGPT levels, which may be a sign of harm to the liver cells or fat buildup and adipocyte proliferation. The bacterial therapy increased mRNA levels of lipolytic transcription factors, including peroxisome proliferator-activated receptor-α, potentially leading to higher expression of fatty acid oxidation genes like acyl-CoA oxidase and carnitine palmitoyltransferase- 1. Alongside, the down-regulation of the genes for sterol-regulatory elementbinding protein-1c (2.23-fold), acetyl-CoA carboxylase (2.54-fold), and fatty acid synthase (1.61-fold) represented the simultaneous stoppage of both adipocytogenesis and fatty acid synthesis. E2_MCCKT significantly reduced the expression of pro-inflammatory cytokines [IL-1Ra (1.19-fold) and TNF-α (2.26-fold)] while increasing IL-10 (1.07-fold) in the protein expression investigation. Moreover, the gastroprotective effects of Lp. plantarum E2_MCCKT was investigated using a cold-induced gastric ulcer mice model. Gastric ulcer is a chronic gastrointestinal illness characterized by a significant disruption in the mucosal barrier. The adult male mice (28.4±1.62 g) were randomly divided into three groups (n = 10): normal (N) group, positive control (PC) group, and Lp. plantarum E2_MCCKT treatment (T) group. The oral supplementation of the Lp. plantarum E2_MCCKT strain (109 cells daily with a standard diet) significantly reduced cold-induced gastric erosion in the stomach mucosa after 30 days. The histopathological study clearly showed that probiotic Lp. plantarum E2_MCCKT strain could protect the stomach epithelial cells from cold injury. Moreover, Lp. plantarum E2_MCCKT strain might regulate relative mRNA expressions corresponding to gastric inflammation, such as up-regulation of IL-10 (2.31-fold) and down-regulation of IFN-γ (2.17-fold), IFN-λ (10.05±0.03-fold), and IL- 12 (1.31-fold). In the context of protein expression study, Lp. plantarum E2_MCCKT significantly increased IL-10 (1.06-fold) and PPAR-α (1.13-fold) expression while decreasing inflammatory cytokines [IL-6 (1.18-fold), and PPAR-γ (1.11-fold)] expression compared to PC group, which indicated the anti-inflammatory activity of the strain during cold-induced gastric ulcer. In conclusion, this study highlighted the promising probiotic potential of Lp. plantarum E2_MCCKT, in combating obesity and gastric ulcers. In vitro analysis showed strong survival in acidic and bile salt conditions and beneficial autoaggregation and hydrophobicity properties. In vivo studies in obese mice indicated that Lp. plantarum E2_MCCKT reduced weight gain, improved lipid metabolism, and lowered inflammation by modulating relevant transcription factors and inflammatory markers. A gastric ulcer model also demonstrated significant protection by reducing gastric erosion and inflammation. Hence, the probiotic Lp. plantarum E2_MCCKT might be an alternative therapeutic agent for obesity and cold-induced gastric ulcer management. Tue, 24 Mar 2026 00:00:00 GMT https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/893 2026-03-24T00:00:00Z https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/892 Establishment of Anti-Obesity and Anti-Inflammatory Potentiality of Conjugated Fatty Acid Isolated from Marine Phasa Fish (Setipinna Phasa) Oil on In-Vivo and In-Vitro Model. Panchali, Titli Obesity is a major public health problem day by day due to unhealthy eating habit. The buildup of an excessive quantity of bodily fat is the complex condition known as obesity. It is a condition that develops when energy intake and expenditure are out of balance. Inflammation and hypertrophy are caused by storage of too much white adipose tissue resulting adiposity, which also secreted several pro-inflammatory cytokines. Several marketed drugs of obesity management have many side effects for long time ingestion. Anchovy, Gangetic Hairfin despite being a fresh water fish and going by the name “Phasa,” (Setipinna phasa), is thought to be able to tolerate some saline in the water. Basically, it can be found in Orissa’s River and estuaries as well as the Ganges system. The fish is easily available, low cost, and popular in West Bengal and Orissa. Considering this, it is aimed to establish the anti-obesity effect of marine Phasa fish (Setipinna phasa) oil against obese model. For the establishment of this research, Extraction, Isolation of Setipinna phasa fish oil were done by FAME (Fatty Acid Methyl Extraction) procedure and characterization of fish oil extracted from Setipinna phasa with a gas chromatography mass spectrometer and to check the edibility of fish oil physicochemical characteristics were done. Establishment of anti-obesity and anti-inflammatory effect of fatty acid isolated from marine Setipinna phasa fish oil on in-vitro model. Here I have chosen 3T3-L1 preadipocyte cell line for this study. I have treated the cell with 11,14,17-ITA. Investigation of the anti-obesity and anti- inflammatory effect of marine Setipinna phasa fish oil on high fat diet induced in-vivo model: selection of most effective dose of Setipinna phasa fish oil. For the investigation of the anti- obesity and anti-inflammatory effect of marine Setipinna phasa fish oil on high fat diet induced in-vivo model: A time dependent study of Setipinna phasa fish oil to select most effective duration. Establishment the mechanism of anti-obesity effect of isolated fatty acid through transcriptional and translational alteration: a dose dependent study of 11,14,17-ITA were done in male albino mice. By using GC-MS, several fatty acids were characterized from Setipinna phasa oil like EPA, DHA, methyl linolenate, hexadec-9-enoic acid, and octadec- 11-enoic acid. A GC-MS investigation investigated the existence of fatty acids that are good for human health as well as obesity and for evaluation the anti-obesity effect different parameters would be considered as Body weight, total lipid profile and different obesity and obesity associated inflammatory markers. The Physicochemical characteristics of Phasa fish oil revealed that oil quality was good. Application of Phasa fish oil on high fat diet induced obese mice significantly reduced body weight, serum lipid profile compared to obese group. Several adipocytokines are downregulated and upregulated which indicated the potentiality of this oil and essential fatty acid. From this study it was also demonstrated that the pathway of the fatty acid and marine fish oil exactly worked as anti-obesity. It is much conspicuous that Phasa fish oil, enriched with essential fatty acid might be used as an anti-obese and anti-inflammatory supplement. Tue, 24 Mar 2026 00:00:00 GMT https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/892 2026-03-24T00:00:00Z