https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/877 2026-05-02T00:17:07Z 2026-05-02T00:17:07Z Das, Tridip Kumar https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/893 2026-04-17T18:30:55Z 2026-03-24T00:00:00Z

Anti-Obesity and Anti-Gastric Ulcer Activity of Probiotic Lactic Acid Bacterium Isolated from Traditional Fermented Foods. Das, Tridip Kumar Lactic acid bacteria (LAB) have garnered significant attention for their remarkable probiotic potential, which plays a vital role in promoting gut health and enhancing overall well-being. Beneficial microorganisms, particularly LAB, are primarily present in traditional fermented foods, which represent a rich source of diverse strains of LAB. Each strain possesses distinct functional properties that can positively influence human health. The aim of this study was to evaluate the anti-obesity and anti-gastric ulcer activity of probiotic lactic acid bacterium isolated from traditional fermented foods. Considering this, a comprehensive isolation process was conducted, resulting in the isolation of 12 distinct lactic acid bacterial colonies from a variety of fermented products, including idli batter, dosa batter, and haria (a rice-based fermented beverage). The standard plate method was employed for LAB isolation using Rogosa SL agar media (supplemented with 0.132% acetic acid). The morphological analysis confirmed that all strains were rod-shaped, Gram-positive, non-motile, and non-spore-forming. The negative test for Indole, Methyl Red, Voges-Proskauer, and citrate utilization confirmed their reliance on carbohydrate fermentation. The enzymatic analysis revealed that the isolates might exhibit significant α-amylase, glucoamylase, and protease activity. Further in vitro probiotic characterization of the isolated LAB was conducted. Based on the cumulative probiotic score, we selected the E2_MCCKT strain for further investigation. After 120 min of incubation, the E2_MCCKT strain exhibited the highest survivability at different pH levels. The survival rate of the E2_MCCKT strain was 98.4% at pH 2, 99.3% at pH 3, and 100% at pH 6.8, which was statistically nonsignificant. Similarly, the E2_MCCKT strain demonstrated significant resistance to bile salts and could endure 0.3% and 2% bile salt concentrations for 120 min. The survival rate of the strain was 98.8% in 0.3% bile and 97.69% in 2% bile salt after 120 min of incubation. Concurrently, the E2_MCCKT strain exhibited moderate autoaggregation ability (52.52%) and surface hydrophobicity (38.08%), which was higher in comparison to other isolates. The antibiotic susceptibility tests indicated that the bacterium had intermediate sensitivity to ampicillin and chloramphenicol. The bacterium was identified as Lactiplantibacillus plantarum E2_MCCKT through 16S rRNA gene sequence analysis. E2_MCCKT exhibited non-haemolytic activity, making it a safe candidate for probiotic applications. Subsequently, E2_MCCKT was tested on obese mice to demonstrate its potential anti-obesity effects. The initial body weight for all male albino mice was 15.39±0.19g, and they were acclimated for 10 days in standard conditions (32±2°C and 50% humidity) with a 12-hour light/dark cycle. The mice were provided with regular food and free access to water. Mice were randomly selected and divided into three groups (n=5) based on the food and treatment. The first group, known as the normal diet (ND), was fed a meal consisting of foods with a ratio of carbohydrate, protein, and fat (64.2:22.3:13.5). The second group was the high-fat diet (HFD), which received lab-made food with a ratio of carbohydrates, protein, and fat (38.9:22.2:38.9). The high-fat treatment group (HFT) was the final group; it received a highfat diet for 8 weeks and probiotic Lp. plantarum E2_MCCKT (109 CFU/ml) for the final 4 weeks. The HFT group received treatment for the probiotic bacteria four weeks following the onset of obesity. The final average body weight and BMI were found to be high in HFD mice after the eightweek investigation period. Furthermore, the HFD group had the greatest levels of total serum cholesterol, triglycerides, LDL, and VLDL when compared to the other groups. In contrast, the Lp. plantarum E2_MCCKT treatment was able to reduce these parameters drastically, and the results were comparable to those of the ND group. The HFD group had higher SGOT and SGPT levels, which may be a sign of harm to the liver cells or fat buildup and adipocyte proliferation. The bacterial therapy increased mRNA levels of lipolytic transcription factors, including peroxisome proliferator-activated receptor-α, potentially leading to higher expression of fatty acid oxidation genes like acyl-CoA oxidase and carnitine palmitoyltransferase- 1. Alongside, the down-regulation of the genes for sterol-regulatory elementbinding protein-1c (2.23-fold), acetyl-CoA carboxylase (2.54-fold), and fatty acid synthase (1.61-fold) represented the simultaneous stoppage of both adipocytogenesis and fatty acid synthesis. E2_MCCKT significantly reduced the expression of pro-inflammatory cytokines [IL-1Ra (1.19-fold) and TNF-α (2.26-fold)] while increasing IL-10 (1.07-fold) in the protein expression investigation. Moreover, the gastroprotective effects of Lp. plantarum E2_MCCKT was investigated using a cold-induced gastric ulcer mice model. Gastric ulcer is a chronic gastrointestinal illness characterized by a significant disruption in the mucosal barrier. The adult male mice (28.4±1.62 g) were randomly divided into three groups (n = 10): normal (N) group, positive control (PC) group, and Lp. plantarum E2_MCCKT treatment (T) group. The oral supplementation of the Lp. plantarum E2_MCCKT strain (109 cells daily with a standard diet) significantly reduced cold-induced gastric erosion in the stomach mucosa after 30 days. The histopathological study clearly showed that probiotic Lp. plantarum E2_MCCKT strain could protect the stomach epithelial cells from cold injury. Moreover, Lp. plantarum E2_MCCKT strain might regulate relative mRNA expressions corresponding to gastric inflammation, such as up-regulation of IL-10 (2.31-fold) and down-regulation of IFN-γ (2.17-fold), IFN-λ (10.05±0.03-fold), and IL- 12 (1.31-fold). In the context of protein expression study, Lp. plantarum E2_MCCKT significantly increased IL-10 (1.06-fold) and PPAR-α (1.13-fold) expression while decreasing inflammatory cytokines [IL-6 (1.18-fold), and PPAR-γ (1.11-fold)] expression compared to PC group, which indicated the anti-inflammatory activity of the strain during cold-induced gastric ulcer. In conclusion, this study highlighted the promising probiotic potential of Lp. plantarum E2_MCCKT, in combating obesity and gastric ulcers. In vitro analysis showed strong survival in acidic and bile salt conditions and beneficial autoaggregation and hydrophobicity properties. In vivo studies in obese mice indicated that Lp. plantarum E2_MCCKT reduced weight gain, improved lipid metabolism, and lowered inflammation by modulating relevant transcription factors and inflammatory markers. A gastric ulcer model also demonstrated significant protection by reducing gastric erosion and inflammation. Hence, the probiotic Lp. plantarum E2_MCCKT might be an alternative therapeutic agent for obesity and cold-induced gastric ulcer management.

2026-03-24T00:00:00Z Panchali, Titli https://mcc-idr.l2c2academy.co.in/xmlui/handle/123456789/892 2026-04-17T18:30:53Z 2026-03-24T00:00:00Z

Establishment of Anti-Obesity and Anti-Inflammatory Potentiality of Conjugated Fatty Acid Isolated from Marine Phasa Fish (Setipinna Phasa) Oil on In-Vivo and In-Vitro Model. Panchali, Titli Obesity is a major public health problem day by day due to unhealthy eating habit. The buildup of an excessive quantity of bodily fat is the complex condition known as obesity. It is a condition that develops when energy intake and expenditure are out of balance. Inflammation and hypertrophy are caused by storage of too much white adipose tissue resulting adiposity, which also secreted several pro-inflammatory cytokines. Several marketed drugs of obesity management have many side effects for long time ingestion. Anchovy, Gangetic Hairfin despite being a fresh water fish and going by the name “Phasa,” (Setipinna phasa), is thought to be able to tolerate some saline in the water. Basically, it can be found in Orissa’s River and estuaries as well as the Ganges system. The fish is easily available, low cost, and popular in West Bengal and Orissa. Considering this, it is aimed to establish the anti-obesity effect of marine Phasa fish (Setipinna phasa) oil against obese model. For the establishment of this research, Extraction, Isolation of Setipinna phasa fish oil were done by FAME (Fatty Acid Methyl Extraction) procedure and characterization of fish oil extracted from Setipinna phasa with a gas chromatography mass spectrometer and to check the edibility of fish oil physicochemical characteristics were done. Establishment of anti-obesity and anti-inflammatory effect of fatty acid isolated from marine Setipinna phasa fish oil on in-vitro model. Here I have chosen 3T3-L1 preadipocyte cell line for this study. I have treated the cell with 11,14,17-ITA. Investigation of the anti-obesity and anti- inflammatory effect of marine Setipinna phasa fish oil on high fat diet induced in-vivo model: selection of most effective dose of Setipinna phasa fish oil. For the investigation of the anti- obesity and anti-inflammatory effect of marine Setipinna phasa fish oil on high fat diet induced in-vivo model: A time dependent study of Setipinna phasa fish oil to select most effective duration. Establishment the mechanism of anti-obesity effect of isolated fatty acid through transcriptional and translational alteration: a dose dependent study of 11,14,17-ITA were done in male albino mice. By using GC-MS, several fatty acids were characterized from Setipinna phasa oil like EPA, DHA, methyl linolenate, hexadec-9-enoic acid, and octadec- 11-enoic acid. A GC-MS investigation investigated the existence of fatty acids that are good for human health as well as obesity and for evaluation the anti-obesity effect different parameters would be considered as Body weight, total lipid profile and different obesity and obesity associated inflammatory markers. The Physicochemical characteristics of Phasa fish oil revealed that oil quality was good. Application of Phasa fish oil on high fat diet induced obese mice significantly reduced body weight, serum lipid profile compared to obese group. Several adipocytokines are downregulated and upregulated which indicated the potentiality of this oil and essential fatty acid. From this study it was also demonstrated that the pathway of the fatty acid and marine fish oil exactly worked as anti-obesity. It is much conspicuous that Phasa fish oil, enriched with essential fatty acid might be used as an anti-obese and anti-inflammatory supplement.

2026-03-24T00:00:00Z